Reproduction Abstracts

One study showed increased fertilization in IVF when added seminal plasma of bulls with high fertility rates in semen of low fertile. The aim of the present study was to evaluate the difference of addition homologous and heterologous seminal on donkey frozen semen. Five ejaculates from two donkeys were used. Semen was diluted (1:1) with a milk-based extender (Botu-Semen). The semen was frozen with Botu-Cryo extender in isothermal box in straws containing 100×106</sup...

Introduction: Pro-inflammatory cytokines are important mediators of the early phases of gestation. Among all these cytokines, the macrophage migration inhibitory factor (MIF) plays significant roles in pregnancy establishment. In many cell types, low concentrations of glucocorticoids (GCs) are known to enhance MIF secretion, but the effect of GCs on endometrial cells has not been elucidated. Bisphenol A (BPA) is an environmental chemical that can alter reproductive physiology ...

We report the effect of allopregnanolone (ALLO, 3α-hydroxy-5α-pregnan-20-one), on morphological changes and on angiogenesis in the rat ovary. ALLO, one of the best characterized neurosteroids, has effects on the reproductive female biology. First, we previously demonstrated that ALLO inhibited ovulation, secretion of LH and progesterone, increased the prolactin levels, inhibited the female receptivity and apoptosis in rat corpus luteum (CL). Now, we show the effect o...

Pulsatile secretion of GnRH/LH is indispensable for puberty onset and normal reproductive functions in mammalian species. A cohort of neurons expressing three neuropeptides, kisspeptin, neurokinin B (NKB), and dynorphin (KNDy neurons), localized in the hypothalamic arcuate nucleus (ARC), are considered to be a source of GnRH pulse generator. A synchronous discharge of KNDy neurons might be obligatory for pulsatile GnRH secretion. The present study aimed to determine whether NK...

Background: As a consequence of cryopreservation of human ovaries preformed in our laboratory we have access to human small antral follicles.Material: Human granulosa cells (GC) from individual small antral follicles (4–6 mm in diameter) were spilt in two and either cultured or snap-frozen immediately after isolation from the follicular fluid. Microarray analysis was used on both the cultured and the non-cultured sample, to examine their gene expres...

Catsper is a Ca2+ permeable ion channel in the principal piece of the sperm flagellum that is crucial in regulation of motility. It is multimodally sensitive, being activated by voltage, pHi and diverse agonists including progesterone. Elevation of pHi and progesterone both shift voltage-sensitivity of the channel to more negative potentials and thus may act synergistically to enhance Ca2+-influx.1 However, intracellular alkalinisation of human...

Introduction: Cyclic ovarian function involves continual tissue remodelling. During ovulation macrophages invade the ovary and secret pro-inflammatory cytokines such us TNF- and IL6 that have local actions on ovarian cells. Here we investigated i) the effect of macrophages on secretion of oestradiol by granulosa cells (TC) and androstenedione by theca cells (TC) and ii) the effect of macrophages on TC and stroma cell (SC) migration was also assessed using an in vitro ...

Lysophosphatidic acid (LPA) is a bioactive lipid mediator that exerts a wide range of biological actions. LPA acting specifically through LPA receptor 1 (LPAR1) seems to be essential for the development of fibrosis in several organs. We determined whether the LPA–LPARs signalling system correlates to equine endometrial fibrosis (endometrosis). A total of 24 uteri from diestrus (n=6 for each category: I, II A, II B, and III according to Kenney and Doig classificat...

Programmed necrosis or necroptosis is an alternative form of cell death that is regulated by a caspase-independent pathway. The aim of the study was to determine if necroptosis participate in bovine CL luteolysis. In Experiment 1. the RIPK1 and RIPK3 mRNA transcription was determined in bovine i) CLs from early, developing, mid, late and regressed-stage (n=4 for each stage), ii) CLs after colpotomy collected within 0-, 2-, 4- and 12-h (each n</em...

Reproductive ageing in females is associated with reduced oocyte maturation potential and developmental competence. Oocyte chromatin configuration and metabolism, have recently been established as correlates of oocyte developmental competence in a number of species. Here, we have evaluated the effect of reproductive ageing on oocyte chromatin morphology and amino acid turnover in germinal vesicle (GV)-staged, ovine oocytes.Cumulus–oocyte complexes w...