ra0001p327 | (1) | WCRB2014
Suetomi Yuta
, Tsukamura Hiroko
, Ohkura Satoshi
, Matsuda Fuko
Neurons essential for reproductive functions are distributed in hypothalamus. The precise functions of these neurons are not fully analyzed at a cellular level, especially in domestic animals. To analyze cellular mechanisms that control ruminant reproduction, we generated neuronal cell lines by immortalizing fetal goat hypothalamus.The hypothalamic arcuate nucleus was collected from a female Shiba goat at E118. The tissue was dispersed and plated. Twenty...