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Reproduction Abstracts

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ra0001p064 | (1) | WCRB2014

The effects of PHE mixture, theophylline, and sperm concentrations on fertilization and development of bovine oocytes in vitro

Kang Sung-Sik , Yanagawa Yojiro , Nagano Masashi

Introduction: We aimed to establish an efficient in vitro fertilization (IVF) system without optimization of sperm concentration of individual bulls, which can obtain stable fertilization and development. We examined the effects of PHE mixture (20 μM D-penicillamine, 10 μM hypotaurine, and 1 μM epinephrine), theophylline (2.5 mM), and sperm concentrations (1, 2, or 5×106 spermatozoa/ml) on fertilization and development of bovine oocytes ...
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Published by BioScientifica

Reproduction Abstracts
ISSN 2052-1472 (online)
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