Reproduction Abstracts

Although mammalian embryo culture is important for research in early development as well as reproduction engineering, rat embryo culture is still infant other than that of mice. The aim of the present study was to improve rat embryo culture in vitro, we compared modified rat-one cell embryo cuture medium (R1ECM) with potassium simplex optimized medium with amino acids (KSOMaa) and that effect of addition of EDTA (0.013 mM) and glutamine (1 mM) to mR1ECM on develop to ...

Although GH receptor (GHR) gene deletion causes a hereditary dwarfism and also shows a longer lifespan, the fact was shown only in mice. Because it was difficult to generate knockout (KO) animals other than mice. Recently, the generation of KO animals became possible without difficulty due to advances in genome editing technologies such as transcription activator-like effector nuclease (TALEN). In the present study, we examined the effect of cRNA concentration microinjected cy...