ra0001p245 | (1) | WCRB2014
Song Hyuk
, Lee Won Young
, Lee Kyung Hoon
Introduction: It is necessary to establish and culture of spermatogonia stem cells (SSCs) in vitro to perform the meiotic differentiation study and to generate transgenic spermatozoa in vitro or ex vivo. This study was focused on in vitro derivation and establishment of SSCs from porcine and canine.Materials and methods: Five day-old porcine and 2-month-old canine testes were used to isolated SSCs and testicular somati...