Reproduction Abstracts

This study was designed to investigate whether acteoside (antioxidant) improves canine SCNT efficiency. Cell cycle of canine fetal fibroblasts was synchronized by culturing to contact inhibition, serum starvation and treating with acteoside. Cell cycle synchronization, apoptosis and reactive oxygen species (ROS) detection were analyzed using flow cytometry. The fibroblasts, prepared by confluent-cell culture or treating with 30 μM acteoside for 48 h, were reconstructed in...