Reproduction Abstracts

Heat stress can impair ovarian function and reduce fertility in cattle. It is correlated with oxidative stress and can induce granulosa cell apoptosis and reduce steroid production. Effects of heat stress can be reversed by melatonin, but studies with ovarian cells have only been evaluated under atmospheric oxygen tension. Here we cultured granulosa cells (GCs) from antral follicles in fibronectin-coated 12-well plates in M199 for up to 144h under physiological (5%) oxygen ten...