ra0001p081 | (1) | WCRB2014
Malaweera Don Buddika Oshadi
, Ramachandra Sisitha
, Kim Ghangyong
, Kim Seokjoong
, Cho Jongki
To investigate the efficient laboratory techniques for the injection of DNA, pEGFP-N1 commercial plasmid were microinjected into porcine parthenogenetic and IVF embryos to explore the injection time, volume and concentration, for the efficient blastocyst production. In experiment 1, to investigate injection time, compared four different time durations (2, 4, 6 and 8 h) after post activation and 6 h of co-incubation with sperms. There were no significant difference (P&...