Reproduction Abstracts

To determine whether chromosomes in porcine first polar body (PB1) can complete the second meiotic division and participate in subsequent normal embryo development, we examined developed competence of chromosome in PB1 that had been injected into enucleated MII stage oocytes by nuclear transfer method (chromosomes replacement group, CR group). After parthenogenetic activation (PA) and in vitro fertilization (IVF), the cleavage rate of reconstructed oocytes in IVF grou...

Introduction: Accurate measurement of ovulation timing is difficult in the dog. In this study, we investigate the expression of steroid hormone receptors in the ovary and oviduct before and after ovulation in the dog.Material and methods: Estrus and ovulation period were measured by serum concentrations of progesterone hormone and LH. LH surge was defined as day 0. The ovary and oviduct were isolated at the day before LH surge (day-1), 2 day after ovulat...

Somatic cell nuclear transfer (SCNT) in dogs has been used in vivo-matured oocytes due to low efficiency of in vitro maturation system. Actually, more information is needed to acquire good quality in vivo oocytes for the dog research. The objective of present study was to evaluate whether the progesterone (P4) on estimated ovulation time and the parity of donor bitches have any effect on the maturity of oocytes. In vivo oocytes were retrieve...