Reproduction Abstracts

Lysophosphatidic acid (LPA) is a simple phospholipid and exerts various biological functions effecting on reproductive processes in rats, pigs, ewes and cow. LPA acts through three subtypes of Edg family G protein-coupled receptors (LPA1-3) and ‘non-Edg family’ LPA receptors (LPA4-6). The aim of the study was to determine LPA: i) concentrations and its receptors mRNA transcription, and ii) effect on P4 secretion from equine CL during the oestrous cycle and...

Introduction: In the absence of pregnancy, the resumption of a new estrous cycle is assured by functional and structural regression of the corpus luteum (CL). We have recently characterized the involvement of Nodal, a morphogen from transforming growth factor beta superfamily, on functional luteolysis in the mare (Galvao et al, 2015 Endocrinology, 157:858–871). Also, tumor necrosis factor α (TNF) has been reported to mediate both functional and structural luteal regr...

The increasing exposure to environmental chemicals is a burden for human reproduction, and can alter endometrial functions and maternal–embryo interactions. The available in vitro endometrial models often fail to represent the complexity of cellular environment, cells shape and organization, and could give misleading results on the effects of environmental chemicals. Here, a tissue-engineered (3D) decidua was used as a toxicological model. Human stromal cells wer...

Programmed necrosis or necroptosis is an alternative form of cell death that is regulated by a caspase-independent pathway. The aim of the study was to determine if necroptosis participate in bovine CL luteolysis. In Experiment 1. the RIPK1 and RIPK3 mRNA transcription was determined in bovine i) CLs from early, developing, mid, late and regressed-stage (n=4 for each stage), ii) CLs after colpotomy collected within 0-, 2-, 4- and 12-h (each n</em...