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ra0001p157 | (1) | WCRB2014

A high concentration of polyvinylpyrrolidone in culture medium affects the number of tranzonal projections in bovine oocyte–granulosa cell complexes in long-term culture

Hirao Yuji , Somfai Tamas , Akagi Satoshi , Haraguchi Seiki , Watanabe Shinya

Introduction: A high concentration of polyvinylpyrrolidone (PVP, average molecular weight 360 000) supplementation in culture medium exerts a positive influence on the growth and morphological organization of bovine oocytecumulus/granulosa cell complexes in a long-term culture. The objective of the present study was to determine the effect of PVP supplementation on the number of transzonal projections (TZPs) traversing the zona pellucida from cumulus cells to the oocyte....

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A high concentration of polyvinylpyrrolidone in culture medium affects the number of tranzonal projections in bovine oocyte–granulosa cell complexes in long-term culture

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