Reproduction Abstracts

Somatic cell nuclear transfer (SCNT) has a broad spectrum of many potential applications but the efficiency is still disappointingly low. It is known that major cause of it, is incorrect or incomplete reprogramming of the transferred donor nuclei.Many report indicates that Wnt/β-catenin signaling pathway plays a pivotal role in the process of somatic cell reprogramming. As CHIR is known as one of the activators of Wnt pathway by inhibiting glycogen ...

Oct4 and Nanog are the transcription factors responsible for pluripotency maintenance in embryonic stem cells (ESCs), and activation of these genes in differentiated cells is considered the earliest signal of nuclear reprogramming. To date, nuclear reprogramming in somatic cells can be induced by nuclear transfer, induced expression of defined factors (Oct4, c-myc, Sox-2, and Kfl4) and, more recently, by a short exposure to a low pH. However, work in salamander as well as in p...