SRF2015 SYMPOSIA SRF-SRB Exchange Lecture (1 abstracts)
SRF–SRB Exchange Lecture: Musashi RNA binding protein MSI2 interacts with SFPQ and controls the expression of target mRNAs Tbx1 and Piwil1 in male germ cells during spermatogenesis
Jessie M Sutherland 1 , Barbara A Fraser 1 , Alexander P Sobinoff 1 , Nicole A Siddall 2 , Peter Koopman 3 , Gary R Hime 2 & Eileen A McLaughlin 1
1University of Newcastle, Callaghan, New South Wales, Australia; 2University of Melbourne, Parkville, Victoria, Australia; 3The University of Queensland, Brisbane, Queensland, Australia.
Controlled gene regulation during gamete development is vital for maintaining reproductive potential. During the process of gamete development, male germ cells experience extended periods of inactive transcription despite requirements for continued growth and differentiation. Spermatogenesis therefore provides an ideal model to study the effects of post-transcriptional control on gene regulation. During spermatogenesis post-transcriptional regulation is orchestrated by abundantly expressed RNA-binding proteins (RBPs). One such group of RBPs is the Musashi family, previously identified as critical regulators of testis germ cell development and meiosis in Drosophila, and also shown to be vital to sperm development and reproductive potential in the mouse. Herein, we focus in depth on the role and function of the vertebrate Musashi ortholog: Musashi-2 (MSI2).
Through detailed expression studies and utilising our novel transgenic Msi2 testis-specific over-expression model we have identified two unique RNA-binding targets of MSI2 in spermatogonia, Piwil1 and Tbx1, and have demonstrated a role for MSI2 in the regulation of mature mRNA localisation and expression. We have also provided evidence to suggest that splicing protein, SFPQ, acts in complex with MSI2, exclusively in the nucleus of spermatocytes and spermatids, functioning in pre-mRNA processing. This firmly establishes MSI2 as a master regulator of post-transcriptional control during post-mitotic spermatogenesis and highlights the significance of the sub-cellular expression of RNA binding proteins in relation to their function.
Volume 2
Article tools
My recent searches
My recently viewed abstracts
Authors
Reproduction Abstracts
ISSN 2052-1472 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more