Searchable abstracts of presentations at key conferences on reproductive biology and medicine
Reproduction Abstracts (2015) 2 P022 | DOI: 10.1530/repabs.2.P022

SRF2015 POSTER PRESENTATIONS (1) (56 abstracts)

Role of sperm thiols? redox status in keeping rat sperm quiescent in cauda epididymis

Santosh Kumar Yadav , Lokesh Kumar , Aastha Pandey , Bhavana Kushwaha , Jagdamba Prasad Maikhuri & Gopal Gupta


CSIR-Central Drug Research Institute, Lucknow, India.


Background: The sperm require energy-intensive motility to reach the female gamete for delivering the male genome. This energy is conserved by keeping the sperm quiescent in cauda epididymis before ejaculation, but the molecular mechanisms controlling this unique process remain an enigma. Cauda sperm produce H2O2, and we have attempted to study the redox regulation of sperm motility.

Methods: Quiescent and motile sperm were collected from Sprague Dawley rat cauda epididymides and experimented in vitro. Care was taken to prevent motility initiation of quiescent sperm on isolation from epididymis.

Results: Free thiols on quiescent sperm increased by ~2-fold on motility initiation. Caudal sperm failed to initiate motility in presence of 0.1% sulfhydryl-alkylating agent N-ethylmaleimide (NEM), and when applied vaginally before mating 50 mg NEM prevented pregnancy in rabbits. While ~50% motile-sperm lost complete motility when placed in0.05% H2O2, 50% quiescent-sperm did not initiate any motility at 0.02% H2O2. Similarly, all motile sperm could be immobilized by at least 2.0% H2O2 while none of the quiescent sperm could initiate motility at 0.25% H2O2. However, caudal sperm could initiate good motility at pH (6.85) and viscosity (82 cP) of the caudal semen.

Conclusions: The redox potential of sperm thiols may play a crucial role in rat sperm quiescence and motility initiation. The pH and viscosity of caudal semen may not have a critical effect on sperm motility suppression. Quiescent sperm are more susceptible to these factors than motile sperm.

Volume 2

Society for Reproduction and Fertility Annual Conference 2015

Oxford, UK
20 Jul 2015 - 22 Jul 2015

Society for Reproduction and Fertility 

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