WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
Testicular chemical pollutants alter sperm vitality, motility, and morphology: comparative studies in the human and dog
Andrew Byers 1 , Rebecca Sumner 1 , Holly-May Richardson 1 , Claire Stratford 1 , Thomas Cammack 1 , Rachel Moxon 2 , Mathew Tomlinson 3 , Asad Naeem 4 , Jim Craigon 1 , Gary C W England 5 & Richard Lea 1
1University of Nottingham, Loughborough, UK; 2Guide Dogs for the Blind Association, Bishop’s Tachbrook, Leamington Spa, UK; 3Fertility Unit, Nottingham, UK; 4AIR University, Islamabad, Pakistan; 5The University of Nottingham, Sutton Bonington, UK.
Introduction: Declining human and canine male fertility has been associated with exposure to environmental chemicals (ECs). We investigated the effects of ECs on canine and human sperm vitality, motility, and morphology.
Methods: Mean canine testicular levels (MTL) of PCB153 and DEHP were 0.9±0.14 μg/kg (n=8) and 0.31±0.03 μg/g (n=5) respectively. Spermatozoa from dogs (n=7) and human donations (n=9, four donors: approved by Nottingham Medical School Ethics Committee) were cultured with 0, 2×, 10×, 100× MTL of PCB153, DEHP, or mix (PCB153+DEHP). Sperm vitality and morphology were measured (nigrosin eosin/VitalStain: dog/human) at 0 and 240 min. Motility was measured using computer-assisted sperm analysis.
Results and discussion: Dog: PCB153 (2× MTL) and DEHP (independent of concentration) reduced sperm vitality (P=0.049 and P=0.002 respectively). PCB153 increased percent linearity (P=0.036) and straight line velocity (P=0.05) whereas DEHP decreased these parameters (P=0.031 and P=0.0037 respectively). PCB153 increased percent motility (P=0.031) and DEHP decreased percent straightness (P=0.032). Morphology: no effects. Human: Effects on motility inconclusive: number of Cat B sperm increased with DEHP (P<0.001), not Cat A. The proportion of morphologically normal sperm was decreased (P=0.007) and this was confirmed by an increased proportion with abnormal morphology (P=0.007). Treatment effects were primarily due to PCB153. In conclusion, exposure of ejaculated sperm to ECs effects sperm vitality and motility in the dog and sperm morphology in the human. Although the mechanisms underlying these effects and species differences are unknown, they may be associated with declining male fertility.
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Reproduction Abstracts
ISSN 2052-1472 (online)
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