WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
Comparision of DNA fragmentantion and plasma membrane integrity between chilled and frozen semen of bulls
Patricia de Mello Papa 1 , Carlos Ramires Neto 2 , Priscilla Nascimento Guasti 1 , Rosiara Rosaria Dias Maziero 1 , Yame F R Sancler-Silva 3 , Gabriel A Monteiro 3 , Camila P Freitas Dell’aqua 2 , Cassio Renesto Junqueira 1 , Ian Martin 1 , Marco Antonio Alvarenga 1 , Frederico O Papa 3 & Jose Antonio Dell’ Aqua Junior 1
1Paulo State University, Botucatu, Brazil; 2College of Veterinary Medicine and Animal Science, (UNESP)/Univ Estadual Paulista, Botucatu, Brazil; 3UNESP–BOTUCATU, Botucatu, Brazil.
In fixed time artificial insemination (FTAI) is frozen semen is commonly used, however the use of cooled semen is a practice alternative to reduce costs. The aim of this study was compare the difference in DNA fragmentation and plasma membrane integrity between chilled and frozen semen of bulls. Two ejaculates from 20 bulls were collected. Semen was diluted in extender with egg-yolk and glycerol (Botu-Bov™) and divided in two groups: chilled group (CG) samples were cooled at 5 °C for 24 h and frozen group (FG) semen was frozen. Kinect sperm parameters were evaluated by CASA, plasma membrane integrite (PMI, propidium iodate, and FIT–PSA) DNA fragmentation (DNA, acridine orange) by flow cytometry (BD LSRFortessa). The data were compare using T Test. Total motility (%, CG=89.7±0.9a vs FG=76.3±0.1b), progressive motility (%, CG=65.2±1.7a vs FG=58.2±1.2b), percentage of rapid sperm (%, CG=86.0±1.3a vs FG=71.3±1.7b) and PMI (%, CG=76.5±2.2a vs FG=40.7±1.9b) were higher (P<0.05) in CG compare to FG. DNA (%, CG=1.1±0.1a vs FG=1.6±.1b) was lower (P<0.05) in CG than FG. These results showing that chilled semen in bull provides significantly higher sperm kinect parameters and viability and lower DNA fragmentation in comparison with frozen semen. In conclusion, the use of chilled bovine semen is an important alternative in fixed time artificial insemination.
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ISSN 2052-1472 (online)
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