WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
Evaluation of donkey frozen semen quality in different freezing conditions
Pedro Victor de Luna Freire Oliveira 1 , José Victor Oliveira 1 , Yame F R Sancler-Silva 2 , Carlos Ramires Neto 1 , Marcelo L F Oliveira 3 & Frederico O Papa 2
1College of Veterinary Medicine and Animal Science, (UNESP)/Univ Estadual Paulista, Botucatu, Brazil; 2UNESP–BOTUCATU, Botucatu, Brazil; 3University of São Paulo (USP), Pirassununga, Brazil.
There are few studies with donkey semen in the literature. In horses, is related that no difference are in freezing semen using automated systems or Styrofoam box. The aim of the present study was evaluate donkey frozen semen quality in different freezing conditions. Five ejaculates from there donkeys were used. Semen was diluted (1:1) with a milk-based extender. The pellet was resuspended with Botucrio and the straws were packaged. The straws were kept in a refrigerator at 5 °C for 20 min. After this, the samples were divided in: Styrofoam box group (SG) where the straws were placed 6 cm above liquid nitrogen for 20 min and automated system group (AG) where straws were placed in a freezing machine at rate temperature of −60 °C/min until −140 °C. After this, Straws of both groups were immersed in liquid nitrogen. Sperm kinetic analysis by CASA, plasma membrane integrity (MI) by epi-fluorescence microscopy were evaluated. Comparison of sperm parameters was performed by T test. No difference (P>0.05) were observed in total motility (%, SG=75.4±8.2 vs AG=78.0±9.5), progressive motility (%, SG=42.0±8.8 vs AG=45.5±8.5) and MI (%, SG=61.2±10.5 vs AG=63.5±11.8) between the groups. AG had higher trend (0.05a vs AG=101.8±9.7b) and percentage of rapid sperm (%, SG=58.4±12.5a vs AG=64.6±12.1b). The results of this study showed have not difference in frozen donkey semen using Styrofoam box or automated system, however when automated system was using there is higher trend of velocity sperm.
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