WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
Difference between of homologous and heterologous seminal plasma addition on post-thawn donkey frozen semen
Pedro Victor de Luna Freire Oliveira 1 , José Victor Oliveira 1 , Yame F R Sancler-Silva 2 , Carlos Ramires Neto 1 , Camila P Freitas Dell’aqua 1 & Frederico O Papa 2
1College of Veterinary Medicine and Animal Science, (UNESP)/Univ Estadual Paulista, Botucatu, Brazil; 2UNESP–BOTUCATU, Botucatu, Brazil.
One study showed increased fertilization in IVF when added seminal plasma of bulls with high fertility rates in semen of low fertile. The aim of the present study was to evaluate the difference of addition homologous and heterologous seminal on donkey frozen semen. Five ejaculates from two donkeys were used. Semen was diluted (1:1) with a milk-based extender (Botu-Semen). The semen was frozen with Botu-Cryo extender in isothermal box in straws containing 100×106 of total sperm. The samples were thawed at 46 °C for 20 s. After this, the straws of each donkey were divided in two groups: homologous group (G1) the semen was incubated at 37 °C for 5 min with 30% of homologous seminal plasma and heterologous group (G2) the semen was incubated at 37 °C for 5 min with 30% of heterologous seminal plasma. Sperm kinetic analysis (CASA), plasma membrane integrity (MI) by epi-fluorescence microscopy (propidium iodide and 6-carboxyfluorescein diacetate) were evaluated. Comparison of sperm parameters was performed by T test. No difference (P>0.05) were observed in total motility (%, G1=38.0±12.6 vs G2=40.0±6.0), progressive motility (%, G1=18.8±7.5 vs G2=21.5±5.5), progressive angular velocity (μm/s, G1=86.7±6.1 vs G2=88.8±5.3), percentage of rapid sperm (%, G1=24.8±10.5 vs G2=27.8±5.4), and MI (%, G1=32.8±14.3 vs G2=28.3±7.6) between the groups. The results of this study showed have not difference in addition of homologous or heterologous seminal plasma post-thaw on quality of donkey frozen semen.
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