WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
Effect of addition post-thaw of homologous seminal plasma on quality of donkey frozen semen
Pedro Victor de Luna Freire Oliveira 1 , José Victor Oliveira 1 , Carlos Ramires Neto 1 , Yame F R Sancler-Silva 2 , Camila P Freitas Dell’aqua 1 & Frederico O Papa 2
1College of Veterinary Medicine and Animal Science, (UNESP)/Univ Estadual Paulista, Botucatu, Brazil; 2UNESP–BOTUCATU, Botucatu, Brazil.
A recent study showed increase of pregnancy rates in donkey frozen semen with addition of seminal plasma post-thaw. The aim of the present study was to evaluate the effect of addition post-thaw of seminal plasma on donkey frozen semen quality. Five ejaculates from three donkeys were used. Semen was diluted (1:1) with a milk-based extender (Botu-Semen). The semen was frozen with Botu-Cryo extender in isothermal box in straws containing 100×106 of total sperm. The samples were thawed at 46 °C for 20 s. After this, the straws of each stallion were divided in two groups: control group (CG) the semen was incubated at 37 °C for 5 min and plasma seminal group (PG) the semen was incubated at 37 °C for 5 min with 30% of homologous seminal plasma. Sperm kinetic analysis (CASA), plasma membrane integrity (MI) by epi-fluorescence microscopy (propidium iodide and 6-carboxyfluorescein diacetate) were evaluated. Comparison of sperm parameters was performed by T test. Total motility (%, CG=75.4±8.2a vs PG=57.5±16.4b), progressive motility (%, CG=42.0±8.7a vs PG=33.3±13.2b), progressive angular velocity (μ/s, CG=95.8±10.8a vs PG=88.9±10.9b), percentage of rapid sperm (%, CG=58.4±12.5a vs PG=41.0±17.3b), and MI (%, CG=61.2±10.5 vs PG=40.7±19.4) were higher in CG compare to PG. Straightness (%, CG=82.0±1.5a vs PG=87.2±3.0b) and linearity (%, CG=48.5±2.2a vs PG=58.9±4.1b) were higher (P<0.05) in PG group compare to CG. The results of this study showed that addition of homologous seminal plasma post-thaw decrease the sperm kinetic parameters and viability of donkey frozen semen.
Article tools
My recent searches
My recently viewed abstracts
Reproduction Abstracts
ISSN 2052-1472 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more