WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
Invasion of S100β-positive cells into pituitary gland during embryonic period
Hideji Yako 1 , Kotaro Horiguchi 2 , Masashi Higuchi 3 , Ken Fujiwara 4 , Saishu Yoshida 3 , Mo Chen 3 , Kanno Naoko 3 , Hiroki Ueharu 3 , Takako Kato 4 , Takashi Yashiro 4 & Yukio Kato 3
1Division of Life Science, Graduate School of Agriculture, Kawasaki, Japan; 2Department of Health Sciences, Kyorin University, Japan; 3Meiji University, Kanagawa, Japan; 4Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine, Japan.
Introduction: Pituitary cells positive for S100 protein are non-hormone-producing cells and are composed of heterogeneous populations, including folliculo-stellate cell. They are argued for having various functions and are detectable only after birth, but their origin is not yet clarified. Recently, we had detected S100βmRNA in the cDNA library of embryonic pituitary by real-time PCR and observed invasion of non-hormone-producing cells from surrounding mesenchyme into the anterior lobe participating in pituitary vasculogenesis. These data indicate that a possibility of invasion of extra-pituitary precursor of S100-positive cells. This study aimed to verify whether S100-positive cells exist during embryonic period by using the transgenic rat expressing GFP driven by S100β-promoter (S100β-TG).
Materials and methods: S100-TG rat embryonic pituitaries at E15.5 to E20.5 were subjected to immunohistochemistry with various antibodies.
Results and discussion: Immunohistochemistry of the embryonic pituitary at E15.5 demonstrated that GFP-positive cells existed in the mesenchymal cells surrounding the pituitary and at the Atwell’s recess, the intraglandular fossa. Thereafter, they were observed in the parenchyma of the anterior lobe on E18.5. Immunohistochemistry for GFP, PRRX1 (mesenchyme/pituitary-progenitor cell marker) and SOX2 (stem/progenitor cell marker) showed that GFP-positive cells were negative for PRRX1 and SOX2 in the Atwell’s recess on E15.5, followed by conversion to PRRX1-positive/SOX2-negative cells. Further immunostaining with other markers suggested that GFP-positive cells have characters of mesenchymal-, neural crest- and/or vascular-cells. These data indicated that some of S100-positive cells in the pituitary gland are originated from the extra-pituitary cells during embryonic period.
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ISSN 2052-1472 (online)
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