WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
Expression of steriod hormone receptor in the ovary and oviduct during the canine estrus cycle
Byoung-Chul Yang , Jae Gyu Yoo , Dong-Hoon Kim , Dong-Kyo Kim , Hyun-Min Kim , Jin-Gu No , Dong-Hyeon Yeom , Mi-Kyung Choi & Yoon-Seok Nam
National Institute of Animal Science, Rural Development Administration, Suwon, Republic of Korea.
Introduction: Accurate measurement of ovulation timing is difficult in the dog. In this study, we investigate the expression of steroid hormone receptors in the ovary and oviduct before and after ovulation in the dog.
Material and methods: Estrus and ovulation period were measured by serum concentrations of progesterone hormone and LH. LH surge was defined as day 0. The ovary and oviduct were isolated at the day before LH surge (day-1), 2 day after ovulation (day 1) and 5 days after ovulation (day 3) respectively. Real-time RT-PCR was conduct to measure mRNA expression of progesterone receptor (PR), androgen receptor (AR), estrogen receptor alpha (ERα), beta (ERβ), FSH receptor (FSHR), and LH receptor (LHR) mRNA.
Result and discussion: The ERβ is faster than ERα on day 1 was confirmed to be a very highly expressed. Expression of FSHR was very low in the day-1 and day 3 was higher. This is just before the LH surge is extremely lowered the expression of FSHR was found to be higher after ovulation. LHR expression was highly expressed at day-1, days 1 and 3 through and the lower. Most of the results are in oviduct was similar to the results of the ovary, except that there was a little difference in the date only. However, AR and PR showed a very different pattern. Therefore, the steroid hormone receptor mRNA expression was confirmed to be dynamically changing before and after ovulation.
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Reproduction Abstracts
ISSN 2052-1472 (online)
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