WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
DNA methylation analysis in sperm from boars exhibiting normal and altered spermograms
Annabelle Congras 1 , Martine Yerle-Bouissou 1 , Florence Vignoles 1 , Emeline Lhuillier 1 , Sylvain Foissac 1 , Alain Pinton 2 , Stéphane Ferchaud 3 , Olivier Bouchez 1 , Juliette Riquet 1 & Herve Acloque 1
1INRA, Castanet-Tolosan, France; 2ENVT, Toulouse, France; 3INRA, Surgères, France.
In the last decade, more and more studies have reported aberrant pattern of methylation in sperm DNA of patients with an altered spermogram. It is also admitted that epigenetic reprogramming during germ cell development is a key mechanism for the production of functional gametes and the proper development of the embryo. As poor semen quality is also a key issue for farm animals’ productivity, we studied the methylation profile of pig sperm DNA of fertile and poor-quality sperm boars. We first described the methylome of control sperm cells using MeDIP-Seq. We then focused on the methylation level of imprinted genes in poor-quality semen by MeDIP-qPCR analysis, and then perform single base methylation analysis by bisulfite conversion and pyrosequencing in regions of interest. Methylome analysis and comparison with mouse and human data revealed the high conservation of the methylation pattern of sperm cells between mammals: sperm DNA is highly methylated with the exception of CpG islands and promoters. We nevertheless observed some discrepancies between species, like in the promoter of the developmental gene POU5F1 which was highly methylated in human sperm only, suggesting a different dynamics of activation of this gene following fertilization. Global methylation level, assayed by LUMA, did not vary between control and poor-quality sperm DNA but local analysis revealed an increased methylation level in the promoter of NESP55 and GNASXL transcripts of the GNAS locus in some boars presenting an altered spermogram, indicating a possible new role for this locus in the development of efficient gametes.
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Reproduction Abstracts
ISSN 2052-1472 (online)
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