WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
Genome-wide DNA methylation analysis of bovine clones derived from the same donor cells
Masahiro Kaneda 1 , Shinya Watanabe 2 , Makoto Hirako 2 , Satoshi Akagi 2 , Hélène Kiefer 3 , Luc Jouneau 3 , Marie-Laure Martin-Magniette 4 , Sandrine Balzergue 5 & Takashi Nagai 6
1Tokyo University of Agriculture and Technology, Fuchu, Japan; 2NARO Institute of Livestock and Grassland Science, Tsukuba, Japan; 3INRA, Jouy-en-Josas, France; 4INRA, Paris, France; 5INRA, Evry, France; 6Food and Fertilizer Technology Center, Taipei, Taiwan
Introduction: What is nature versus nurture? To what extent do genetic inheritance and non-genetic factors contribute to one’s character? This is one of the oldest issues in psychology. To answer this historic question, cloned animals are good models because they have identical genetic information to the donors. Previous studies indicated that cloned animals showed different coat color patterns, noseprints and characters compared to the donors. However, no molecular work or genome-wide analysis has been done using genetically identical clones. Here we analyzed DNA methylation patterns of cloned cows derived from the same donor cells and compared with those of non-clones, to describe epigenetic differences in the same genetic background (clones) or in a different genetic background (non-clones).
Materials and Methods: Five cloned cows (Japanese Black, age: 68 to 82 months) and six non-cloned cows (Japanese Black, age: 52 to 129 months) were used for the study. Genomic DNA was extracted from various tissues and DNA methylation analysis was carried out by two methods. Imprinted genes H19, PEG3 and XIST were analyzed by bisulfite sequencing method. Genome-wide DNA methylation analysis (in liver tissues only) was carried out by MeDIP-chip (Methylated DNA Immunoprecipitation microarray) method.
Results and Discussion: By bisulfite sequencing experiments, the variation in DNA methylation levels is not different between clones and non-clones in most of all tissues and genes analyzed. However, MeDIP-chip analysis indicated more variability in non-clones, suggesting that genetic variation influences epigenetic differences. The identification and validation of differentially methylated regions are currently under way.
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ISSN 2052-1472 (online)
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