WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
Early development of bovine embryos depends on the cooperative action between oxidative and endoplasmic reticulum stresses
Seung-Bin Yoon , Hae-Jun Yang , Seong-Eun Mun , Pil-Soo Jeong , Seon-A Choi , Young-Ho Park , Bong-Seok Song , Bo-Woong Sim , Ji-Su Kim , Sun-Uk Kim , Kyu-Tae Chang & Sang-Rae Lee
KRIBB, Chungcheongbuk-do, Republic of Korea
The coupling of reactive oxygen species (ROS) and endoplasmic reticulum (ER) stress has been explored using a variety of biological systems, but little is known regarding their role in the early development of mammalian embryos. Here, we demonstrated that early embryonic development of in vitro-produced (IVP) bovine embryos was governed by the cooperative action between ROS and ER stress. Compared to 5% O2 tension, 20% O2 significantly decreased the blastocyst formation rate and cell survival, accompanied with increases in ROS and sXBP-1 transcript levels, which is an ER stress indicator. In a 20% O2 environment, treatment with glutathione (GSH), a ROS scavenger, decreased ROS levels, which resulted in increased blastocyst formation and cell survival rates. Importantly, the levels of sXBP-1 and ER stress-associated transcripts were reduced by GSH treatment during in vitro culture (IVC) of bovine embryos. Consistent with the effects of GSH supplementation, tauroursodeoxycholate (TUDCA), an ER stress inhibitor, improved blastocyst development rates, trophectoderm proportions, and cell survival. Moreover, ROS and sXBP-1 transcript levels were markedly decreased by supplementation of TUDCA into IVC medium (similar to the GSH treatment group), suggestive of the possible mechanism governing the mutual regulation between ROS and ER stress. Based on these results, developmental competence of IVP bovine embryos was highly dependent on the coupled response between oxidative and ER stress. These results increase our understanding of the mechanism(s) governing early embryonic development and may improve strategies for the generation of IVP embryos with high developmental competence.
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Reproduction Abstracts
ISSN 2052-1472 (online)
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