WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
In vivo embryo production in queens treated with deslorelin acetate (Suprelorin)
Camila Louise Ackermann , Leticia Ferrari Crocomo , Eduardo Trevisol , Tatiana Silva Rascado , Rodrigo Volpato , Carlize Lopes , Carlos Renato de Freitas Guaitolini , Talita de Almeida Costa & Maria Denise Lopes
São Paulo State University, Botucatu, Brazil.
Introduction: Reversibility after use of deslorelin acetate as a contraceptive for queens has been described. However no data concerning the ability of in vitro embryo production after treatement was published.
Objective: This study aimed to evaluate the in vitro embryo production in queens treated with deslorelin acetate (Suprelorin).
Methodology: Twenty queens and one tomcat were used. Ten queens were treated with deslorelin 4.7 mg/animal, the other females were not treated. After 6 months of treatment all queens were spayed, the ovaries were kept during 2 h maximum at 4 °C immersed in PBS. The ovaries were placed in petri dishes and sliced for cumulus oocyte complex (COCs) releasing. Grade I COCs were submitted to in vitro maturation, fertilization and the possible zygotes were maturated for 6 days, according to protocol described by previously (Rascado, 2009). The number of morulae and blastocysts produced were counted on day 3 and 8, respectively. No formal analysis was done.
Results: A total of 87 I COC, 50 (57.47%) morula and 18 (36%) blastocysts (four queens did not produced any blastocyst) were obtained from treated group. From control group we obtained 184 I COC, 103 (55.87%) morula and 23 (22.33%) blastocysts. Clearly the number of grade I COC recovered from treated queens was lesser than the control group, however the percentage of morulae obtained was very similar in both groups and the percentage of blastocysts was higher in the treated group. We concluded that ovaries from queens under contraceptive treatment with deslorelin may use for in vitro embryo production with rates similar to non-treated queens, although individual variations should be expected.
Financial Support:FAPESP 2011/23318-0 2012/09002-3.
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