WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
Changes in gene expressions induced by perinatal estrogen related to the brain sexual differentiation in rodents
Youki Watanabe 1 , Mototsugu Sakakibara 1 , Yoshihisa Uenoyama 1 , Shiori Minabe 1 , Chikaya Deura 1 , Sho Nakamura 2 , Kei-ichiro Maeda 2 & Hiroko Tsukamura 1
1Nagoya University, Nagoya, Japan; 2The University of Tokyo, Bunkyo-ku, Japan.
Brain mechanism responsible for LH surge generation shows sexual dimorphism in rodents. The dimorphism is considered to be due to the perinatal estrogen converted from testicular androgen during developing period. The present paper aimed to clarify the mechanisms mediating the estrogen-induced defeminization of LH surge-generating system. The microarray analysis is an attempt to obtain the candidate gene(s) mediating the perinatal estrogen action causing the brain sexual differentiation. Female mice were injected with estradiol benzoate (EB) or vehicle on the day of birth, and the hypothalamus was collected at either 1, 3, 6, 12, or 24 h after the EB injection. More than 100 genes down-regulated by the EB treatment in a biphasic manner peaked at 3 h and 12–24 h after the EB treatment, while 40–70 genes were constantly up-regulated after the treatment. Ptgds, encoding prostaglandin D2 (PGD2) synthase, was chosen for further examination by semiquantitative RT-PCR in the anterior hypothalamus of neonatal male and female rats. Ptgds expression was significantly lower in the anterior hypothalamus of male rats than females at the day of birth. PGD2 have been reported to play a role in neuroprotection, suggesting that Ptgds could be one of the possible candidate genes, which may mediate the effect of perinatal estrogen responsible for sexual differentiation of the LH surge-generating system.
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ISSN 2052-1472 (online)
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