WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
Differential expression of proteins from the human sperm head and its relation to infertility
Binita Basnet Baruah 1 , Srujana Kola 2 , Lokesh Rukmangadachar 1, , Pradeep Chaturvedi 2 & A Srinivasan 2
1All India Institute of Medical Sciences, Guwahati, India; 2AIIMS, New Delhi, India.
Introduction: Male infertility is one of the major challenges in reproduction. The nature of sub-fertility due to male is as complex as that of female and contributes to about 40–50% of infertility cases in human.
Objective: We propose to identify all the protein molecules that differ in the different region of the human spermatozoa, i.e. the head and the tail. The aim of the study was to identify the differentially expressed protein in the head fraction of the both motile and non-motile human spermatozoa samples, which might provide an insight into the cause of infertility.
Material and methods: Semen samples of normal (above 60% sperm motility) and with low sperm motility (below 15% motility) were taken and the sperm cells were separated. Heads were isolated by sucrose density gradient. The head proteins were trypsin digested and subjected to iTRAQ labelling followed by identification by LC/MS.
Results: The identification of these proteins provides us with information that the head of the sperm is a repertoire of binding proteins, catalytic and structural and molecular activity proteins. So the lower expression or absence of these proteins results in infertility due to head structural deformities or incapability release the necessary enzyme or to mediate effective binding with the oocyte.
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ISSN 2052-1472 (online)
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