WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
Effect of ascorbic acid 2-glucoside supplementation on sperm characteristics during semen cryopreservation in Korean native pig
Juncheol Park , Soojin Sa , Sunho Choi , Hyunjong Kim , Kyuho Cho , Joonki Hong , Duwan Kim & Younghwa Kim
National Institute of Animal Science, Cheonan, Republic of Korea.
Ascorbic acid 2-glucoside (AA-2G), a stable ascorbate derivative, is a prominent antioxidant characterized by its stability in an oxidative environment. The objective of this study was to evaluate the effect of AA-2G on sperm motility, viability and acrosome integrity during semen cryopreservation in Korean native pig. Semen samples were diluted into a freezing extender (LEY) containing lactose hen-egg yolk with glycerol and Orvus Es Paste (OEP) and loaded in 0.5 ml plastic straws. In this experiment, each ejaculated semen sample was split into five aliquots and extended with LEY that supplemented with 0, 100, 200, 300, or 400 μM AA-2G. For freezing the semen samples were cooled from 5 to −5 °C at 6 °C/min, held at −5 °C for 30 s while ice crystal formation was induced, then further cooled from −5 to −80 °C at 40 °C/min, and thereafter from −80 to −150 °C at 60 °C/min using programmable semen freezer. To evaluate the post-thaw sperm quality, semen was thawed at 38 °C for 20 s. Sperm quality in terms of motility, viability, and acrosome integrity was improved by addition of AA-2G to freezing extender during semen cryopreservation. In particular, the sperm motility and acrosome integrity at 1 and 2 h of incubation was higher (P<0.05) in the group treated with 300 μM AA-2G during freezing procedure when compared to the control group. The result of this study shows that the addition of AA-2G to the freezing extender (LEY) improves motility and acrosome integrity of frozen–thawed sperm in Korean native pig.
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Reproduction Abstracts
ISSN 2052-1472 (online)
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