WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
The RNA-binding protein Musashi-2 (MSI2) controls mRNA processing and translational regulation via interactions with SFPQ and PIWIL1 during mammalian spermatogenesis
Jessie M Sutherland 1 , Alexander P Sobinoff 1 , Kate Redgrove 1 , Tara-Lynne Davidson 2 , Nicole A Siddall 3 , Peter Koopman 4 , Gary R Hime 3 & Eileen McLaughlin 1
1University of Newcastle, Callaghan, New South Wales, Australia; 2Institute for Molecular Bioscience, Brisbane, Queensland, Australia; 3University of Melbourne, Parkville, Victoria, Australia; 4The University of Queensland, Brisbane, Queensland, Australia.
Introduction: RNA-binding proteins (RBP) are important catalysts of post-transcriptional gene regulation. The RBP MSI2 directs stem cell function and division through established roles in cell cycle dynamics and cell fate determination. During the complex process of mammalian spermatogenesis, the directed uncoupling of transcription and translation is essential for the correct development of male gametes. We have previously established that dysregulation of MSI2 during germ cell maturation is detrimental to sperm development and fertility. In this study we characterise the molecular mechanisms via which MSI2 exercises control of protein expression during spermatogenesis.
Materials and methods: Differential gene (microarray) and protein (iTRAC) expression studies were undertaken to establish the predominant molecular pathways and biological processes affected by up-regulation of MSI2 in a testis-specific MSI2 over-expression mouse model. Immunoprecipitation assays were performed to identify specific MSI2 interactions in post-meiotic round spermatids.
Results and discussion: Of the differentially expressed (DE) genes, the key molecular networks identified were implicated in cell death, cell morphology, cellular growth and proliferation, and the cell cycle. The most relevant biological processes that characterised the DE proteins related to transcriptional control, the cell cycle and cellular proliferation, translation, apoptosis, RNA splicing/processing, and spermatogenesis. Protein-immunoprecipitation identified that MSI2 acts in complex with slicing factor, SFPQ, and piRNA component, PIWIL1, within round spermatids. This data indicates a role for MSI2 in mRNA processing and translational regulation via direct interactions with SFPQ and PIWIL1. And firmly establishes MSI2 as a key regulator of post-meiotic germ cell development.
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ISSN 2052-1472 (online)
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