WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
Generation of rats from vitrified oocytes with surrounding cumulus cells via IVF with cryopreserved sperm
Naomi Kashiwazaki 1 , Katsuyoshi Fujiwara 1 , Tsubasa Kato 2 , M Kamoshita 2 , H Obata 2 , Syouta Takakusa 1 & Junya Ito 1
1Azabu university, Sagamihara-shi, Japan; 2Graduate School of Veterinary Sciences, Azabu University, Kanagawa, Japan.
The aim of the present study was to evaluate fertility of vitrified rat matured-oocytes with surrounding cumulus cells after IVF with cryopreserved sperm. Oocytes with surrounding cumulus cells and cumulus cells denuded oocytes were vitrified with 30% (v/v) ethylene glycol+0.5 M sucrose+20% (v/v) FCS in PBS (PB1) by using Cryotop. After warming, oocytes were co-cultured with fresh or cryopreserved epididymal sperm for 10 h. Although the fertilization (two pronuclei formation: 2PN formation) of vitrified denuded oocytes were not observed after IVF with fresh sperm, some vitrified oocytes with surrounding cumulus cells (32.7±4.3%) were fertilized. Fresh and vitrified oocytes with surrounding cumulus cells (62.9±5.1 and 41.3±5.0% respectively) were also fertilized after IVF with cryopreserved sperm. In addition, the 113 of vitrified oocytes after IVF with cryopreserved sperm were transferred into the oviducts of recipients. As the results, seven live pups derived from vitrified oocytes and cryopreserved sperm were produced. In conclusion, to our knowledge the present study demonstrates for the first time that vitrified rat oocytes can be fertilized in vitro with cryopreserved spermatozoa and that 2PN embryos obtained from cryopreserved gametes via IVF can develop to term after embryo transfer.
Article tools
My recent searches
My recently viewed abstracts
Authors
Reproduction Abstracts
ISSN 2052-1472 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more