Reproduction Abstracts

Introduction: Neonatal bisphenol A (BPA) exposures affect the developing reproductive tract of females, several distinct stages of oogenesis in the developing ovary. Notch signaling also plays an important role during oogenesis. We aimed to explain whether mRNA levels of Notch receptor and ligands are altered following BPA treatment during neonatal days in vitro.

Materials and methods: Wistar rats were sacrificed and ovaries were isolated on postnatal days 0 and 4. Whole ovaries were cultured on floating filters in ovary organ culture medium in a four-well culture plate. Ovaries were treated with %1 DMSO as vehicle, 1 μM BPA as low dose and 10 μM BPA as high dose at P0–P3 (group I) and P4–P7 (group II) time intervals. Following in vitro treatments, mRNA levels of Notch receptors and ligands were determined with qPCR.

Results and discussion: When compared with vehicle treatment in group I, 1 μM BPA treatment was found to enhanced Notch and their ligands gene expressions. In addition 10 μM BPA treatment increased the gene expression levels of Notch receptors and their ligands except for Notch-3. When compared with vehicle treatment in group 2, 1 μM BPA treatment was found to increased Notch-1, Notch-2, Notch-3, Jagged-1, and Jagged-2 but decreased Notch-4. 10 μM BPA treatment also suppressed Jagged-1 and Notch-4 mRNA levels. It was conclueded that neonatal exposure to BPA can impair the early ovary development by disrupting Notch signaling pathway This research was supported by Tübitak (Tovag 1110751).