WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
The effect of development in vitro
Hitomi Obata 1 , Maki Kamoshita 1 , Tsubasa Kato 1 , Junya Ito 2 & Naomi Kashiwazaki 2
1Graduate School of Veterinary Sciences, Azabu University, Kanagawa, Japan; 2Azabu University, Sagamihara-shi, Japan.
Although mammalian embryo culture is important for research in early development as well as reproduction engineering, rat embryo culture is still infant other than that of mice. The aim of the present study was to improve rat embryo culture in vitro, we compared modified rat-one cell embryo cuture medium (R1ECM) with potassium simplex optimized medium with amino acids (KSOMaa) and that effect of addition of EDTA (0.013 mM) and glutamine (1 mM) to mR1ECM on develop to 2 cell stage embryos and blastocysts. Pronuclear embryos were collected at 29 h after hCG injection from oviducts of superovulated females (4–5 weeks old of Wistar) that naturally mated with males (12–24 weeks old of Wistar). The embryos were cultured at 37.5 °C for 120 h. When cultured with mR1ECM or KSOMaa, 2-cell block was observed in embryos cultured in KSOMaa and that no development to blastocysts.
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ISSN 2052-1472 (online)
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