WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
Fingerprints on oocyte and embryo lipid profiles caused by in vitro embryo production system and fatty acid diet supplementation in bovine
Andrés Felipe González Serrano 1 , Christina R Ferreira 2 , Valentina Pirro 3 , Andrea Lucas-Hahn 1 , Julia Heinzmann 1 , Klaus-Gerd Hadeler 1 , Patrick Aldag 1 , Ulrich Baulain 1 , Marion Piechotta 4 , Sven Dänicke 5 , Robert Graham Cooks 2 & Heiner Niemann 1
1Institute of Farm Animal Genetics (FLI), Neustadt, Germany; 2Purdue University, West Lafayette, Indiana, USA; 3University of Turin, Turin, Italy; 4University of Veterinary Hanover, Hannover, Germany; 5Institute of Animal Nutrition (FLI), Braunschweig, Germany.
Introduction: Lipid content of oocytes and early embryos is critical for embryonic and fetal development. Indeed, the success of assisted reproductive techniques (ARTs) such as cryopreservation and in vitro embryo production is heavily affected by the lipid content of preimplantation embryos.
Materials and methods: Lipid profiling of single in vitro (IVTB) and in vivo (IVVB) produced bovine embryos was performed by desorption electrospray ionization mass spectrometry (DESI – MS). The relative abundance of transcripts related to oocyte development capacity (IGF1R and GJA1) and lipid metabolism (FASN, SCAP, SRBP1, and CPT1b) was assessed by RT-qPCR. Similar analyses were performed from oocytes collected from Holstein – Friesian Heifers after long-term supplementation with rumen-protected stearic conjugated linoleic (CLA) and stearic (SA) acid of the daily diet. Follicular fluid and blood samples were collected from supplemented animals for monitoring lipid profiles by gas chromatography (GC).
Results and discussion: Results showed effects of in vitro culture conditions on the lipid profile and gene expression of embryos when comparing to their in vivo counterpart. The rumen-protected fatty acid supplementation experiments showed modification of the lipid profile of oocytes collected from supplemented donors. Oocytes collected from CLA supplemented Heifers showed significant accumulation of triacylglycerols of unsaturated fatty while oocytes from the SA supplemented group accumulated higher amounts of palmitic acid and plasmalogen species. These results pave the way for improving embryo culture conditions and for identifying fertility impairments associated to the female nutrition. Results support the role of the bovine experimental model for improving ARTs technologies in humans and other mammals.
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ISSN 2052-1472 (online)
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