WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
Endocrine responses and ovarian dynamics in goats treated with low dose of investigational metastin/kisspeptin analog TAK-683 in follicular phase
Nahoko Gomi 1 , Natsumi Endo 1 , Satoshi Ohkura 2 , Yoshihiro Wakabayashi 3 , Hisanori Matsui 4 , Hirokazu Matsumoto 4 , Kaori Ishikawa 4 , Akira Tanaka 4 , Tatsuya Watanabe 4 , Hiroaki Okamura 3 & Tomomi Tanaka 1
1Tokyo Univesity of Agriculture and Technology, Fuchu, Japan; 2Nagoya University, Nagoya, Japan; 3National Institute of Agrobiological Sciences, Tsukuba, Japan; 4Takeda Pharmaceutical Company, Fujisawa, Japan.
The aim of the present study was to evaluate ovarian and hormonal responses to follicular phase administration of a metastin/kisspeptin analog, TAK-683, with a low dose, determined as the minimally effective dose for LH stimulation in ovariectomized goats. At 7–10 days after ovulation, goats received a prostaglandin F2α (PGF2α) injection (day 0) followed by 10 days of CIDR-G® treatment. A PGF2α was injected again at CIDR removal. Then, they received 5 μg of TAK-683 (0 h) intravenously (n=5, I.V.) or subcutaneously (n=3, S.C.) at 12 h after CIDR removal. Vehicle was given intravenously in the same manner (n=4, Cont). Blood samples were collected at 10-min (2–6 h), 2-h (6–24 h) or 6-h (24–48 h) I.V. for analysis of endocrine profiles. Ovarian ultrasonographic images were assessed daily to confirm ovulation after the treatment. A surge-like release of LH was immediately observed after the injection in all animals in I.V. (peak time: hours 4.2±0.6, peak concentration: 73.3±27.5 ng/ml) and S.C. (peak time: hours 4.6±0.4, peak concentration: 62.6±23.2 ng/ml) groups. Estradiol concentrations at hours 24 and 30 in I.V. and S.C. groups were significantly lower than those in the Cont group. Ovulation was detected within 3 days after TAK-683 injection in all animals in I.V. and S.C. groups. There was no significant difference in the changes in the luteal diameter and blood progesterone levels after ovulation between I.V. and S.C. groups. These results show that follicular phase administration of the minimally effective dose of TAK-683 to ovariectomized goats induces a surge-like release of LH followed by ovulation in ovary-intact goats.
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ISSN 2052-1472 (online)
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